flexi trace software Search Results


93
Addgene inc aav1 syn flex tva 2a egfp 2a rvg unc vector core addgene
(A) Diagram of monosynaptic retrograde tracing assay using EnvA-coated G-deleted rabies virus expressing mCherry (EnvA-SADΔG-mcherry). G and <t>TVA</t> proteins were expressed in specific INs by the use of Cre mice together with <t>AAV1-FLEX-TVA-2A-EGFP-2A-RVG</t> (for Chx10+ and Vglut3+ INs) or lox-stop-lox-histone GFP-2A-TVA-2A-RVG mice (for Ptf1a+ INs). (B–D) Representative images of CSNs traced from Chx10+ (B), Ptf1a+ (C), and Vglut3+ (D) INs. In the panels, two images were combined to cover medial and laterals region of the cortex. White dotted lines show the cerebral cortex margins. Coronal section: right, medial; left, lateral. Scale bar, 100 μm. (E) Plotting of EnvA-SADΔG-mCherry+ CSNs traced from Chx10+ (red), Ptf1a+ (green), and Vglut3+ (blue) INs. Top view of the cortex. (F) Ratios of EnvA-SADΔG-mCherry+ CSNs traced from Chx10+ (red), Ptf1a+ (green), and Vglut3+ (blue) INs in ML % 1.7 mm, 1.7–2.0 mm, and R2.0 mm to total labeled neurons. Data are represented as mean ± SEM (n = 3). See also Figure S4.
Aav1 Syn Flex Tva 2a Egfp 2a Rvg Unc Vector Core Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Leyard Optoelectronic 3d motion capture system
a , Module crosscorrelograms for sessions in different rooms (A1×B1) at incremental degrees of rotation in rat #26718 (select number of rotations shown here for visualization purposes; see for additional rotations) (as in , right). Asterisks denote overall maximum for each module. b , Maximum correlation between <t>3D</t> stacks of rate maps of sessions A1 and B1 (with cell identity along the z-axis) as a function of rotation for each module in rat #26718. Maximum correlation refers to the highest value within the crosscorrelation matrix at each rotation. Note that the rotation was nearly identical across modules. c , Rotation of each simultaneously recorded module between sessions (38 session pairs in 10 rats, as in ). Lines connect data from a single recording. A fourth module was recorded in 4 session pairs. Its rotation did not differ from the other simultaneously recorded modules . d , Difference in rotation between simultaneously recorded module pairs (n = 104 module pairs, each pair indicated by a circle). Red crosses indicate medians. Dashed gray line at 90° represents chance. e , Gray lines show coherent rotation between sessions for grid, head direction (HD), and border cells (n = 22 experiments). Lines connect data from a single recording.
3d Motion Capture System, supplied by Leyard Optoelectronic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Marburg GmbH edf trace v 1.0 software
a , Module crosscorrelograms for sessions in different rooms (A1×B1) at incremental degrees of rotation in rat #26718 (select number of rotations shown here for visualization purposes; see for additional rotations) (as in , right). Asterisks denote overall maximum for each module. b , Maximum correlation between <t>3D</t> stacks of rate maps of sessions A1 and B1 (with cell identity along the z-axis) as a function of rotation for each module in rat #26718. Maximum correlation refers to the highest value within the crosscorrelation matrix at each rotation. Note that the rotation was nearly identical across modules. c , Rotation of each simultaneously recorded module between sessions (38 session pairs in 10 rats, as in ). Lines connect data from a single recording. A fourth module was recorded in 4 session pairs. Its rotation did not differ from the other simultaneously recorded modules . d , Difference in rotation between simultaneously recorded module pairs (n = 104 module pairs, each pair indicated by a circle). Red crosses indicate medians. Dashed gray line at 90° represents chance. e , Gray lines show coherent rotation between sessions for grid, head direction (HD), and border cells (n = 22 experiments). Lines connect data from a single recording.
Edf Trace V 1.0 Software, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edf trace v 1.0 software/product/Marburg GmbH
Average 90 stars, based on 1 article reviews
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Image Search Results


(A) Diagram of monosynaptic retrograde tracing assay using EnvA-coated G-deleted rabies virus expressing mCherry (EnvA-SADΔG-mcherry). G and TVA proteins were expressed in specific INs by the use of Cre mice together with AAV1-FLEX-TVA-2A-EGFP-2A-RVG (for Chx10+ and Vglut3+ INs) or lox-stop-lox-histone GFP-2A-TVA-2A-RVG mice (for Ptf1a+ INs). (B–D) Representative images of CSNs traced from Chx10+ (B), Ptf1a+ (C), and Vglut3+ (D) INs. In the panels, two images were combined to cover medial and laterals region of the cortex. White dotted lines show the cerebral cortex margins. Coronal section: right, medial; left, lateral. Scale bar, 100 μm. (E) Plotting of EnvA-SADΔG-mCherry+ CSNs traced from Chx10+ (red), Ptf1a+ (green), and Vglut3+ (blue) INs. Top view of the cortex. (F) Ratios of EnvA-SADΔG-mCherry+ CSNs traced from Chx10+ (red), Ptf1a+ (green), and Vglut3+ (blue) INs in ML % 1.7 mm, 1.7–2.0 mm, and R2.0 mm to total labeled neurons. Data are represented as mean ± SEM (n = 3). See also Figure S4.

Journal: Cell reports

Article Title: Corticospinal Circuits from the Sensory and Motor Cortices Differentially Regulate Skilled Movements through Distinct Spinal Interneurons

doi: 10.1016/j.celrep.2018.03.137

Figure Lengend Snippet: (A) Diagram of monosynaptic retrograde tracing assay using EnvA-coated G-deleted rabies virus expressing mCherry (EnvA-SADΔG-mcherry). G and TVA proteins were expressed in specific INs by the use of Cre mice together with AAV1-FLEX-TVA-2A-EGFP-2A-RVG (for Chx10+ and Vglut3+ INs) or lox-stop-lox-histone GFP-2A-TVA-2A-RVG mice (for Ptf1a+ INs). (B–D) Representative images of CSNs traced from Chx10+ (B), Ptf1a+ (C), and Vglut3+ (D) INs. In the panels, two images were combined to cover medial and laterals region of the cortex. White dotted lines show the cerebral cortex margins. Coronal section: right, medial; left, lateral. Scale bar, 100 μm. (E) Plotting of EnvA-SADΔG-mCherry+ CSNs traced from Chx10+ (red), Ptf1a+ (green), and Vglut3+ (blue) INs. Top view of the cortex. (F) Ratios of EnvA-SADΔG-mCherry+ CSNs traced from Chx10+ (red), Ptf1a+ (green), and Vglut3+ (blue) INs in ML % 1.7 mm, 1.7–2.0 mm, and R2.0 mm to total labeled neurons. Data are represented as mean ± SEM (n = 3). See also Figure S4.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sheep polyclonal anti-GFP AbD Serotec 4745–1051; RRID:AB_619712 Rabbit polyclonal anti-GFP Invitrogen {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 ; RRID: AB_221569 Rat monoclonal anti-GFP Nacalai 04404–84; RRID: AB_10013361 Rabbit polyclonal anti-RFP Rockland 600–401-379; RRID: AB_2209751 Rabbit polyclonal anti-DsRed Clontech 632496; RRID: AB_10013483 Rabbit polyclonal anti-PKCγ Santa cruz sc-211; RRID: AB_632234 Rat monoclonal anti-Ctip2 Abcam ab18465; RRID: AB_10015215 Mouse monoclonal anti-NeuN Millipore MAB377; RRID: AB_2298772 Guinea pig polyclonal anti-Vglut1 Millipore AB5905; RRID: AB_2301751 Bacterial and Virus Strains PRV152 L. Enquist (Princeton Univ) N/A PRV614 L. Enquist (Princeton Univ) N/A AAV1-Syn-EGFP-Cre Penn vector core AV-1-PV1848 AAV1-CAG-tdTomato Penn vector core AV-1-PV3365 EnvA-SADΔG-mcherry E. Callaway and Gene Transfer, Targeting and Therapeutics Core (Salk Inst; Osakada et al., 2011 ) Addgene #32636 AAV1-Syn-FLEX-TVA-2A-EGFP-2A-RVG UNC vector core Addgene #52473 AAV1-CAG-ChR2(H134R)-mCherry Penn vector core AV-1–20938M AAV8-hSyn-DIO-hM4D(Gi)-mCherry UNC vector core Addgene #44362 Chemicals, Peptides, and Recombinant Proteins Green Retrobeads IX Lumafluor N/A Biotinylated dextran amine (10,000MW) Invitrogen D-1956 Fluorogold Fluorochrome N/A Clozapine-N-oxide Sigma-Aldrich C0832–5MG Experimental Models: Organisms/Strains Mouse: Rbp4-Cre GENSAT, MMRRC MMRRC:031125-UCD; MGI:4367067 Mouse: En1-Cre A. Joyner (Memorial Sloan Kettering Cancer Center; Kimmel et al., 2000 ) MGI:2446434 Mouse: Chx10-Cre Azim et al., 2014 N/A Mouse: Chat-Cre The Jackson Laboratory JAX# 006410; MGI: 5475195 Mouse: Ptf1a-Cre C. Wright(Vanderbilt Univ.

Techniques: Retrograde Tracing, Expressing, Labeling

Highlights

Journal: Cell reports

Article Title: Corticospinal Circuits from the Sensory and Motor Cortices Differentially Regulate Skilled Movements through Distinct Spinal Interneurons

doi: 10.1016/j.celrep.2018.03.137

Figure Lengend Snippet: Highlights

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sheep polyclonal anti-GFP AbD Serotec 4745–1051; RRID:AB_619712 Rabbit polyclonal anti-GFP Invitrogen {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 ; RRID: AB_221569 Rat monoclonal anti-GFP Nacalai 04404–84; RRID: AB_10013361 Rabbit polyclonal anti-RFP Rockland 600–401-379; RRID: AB_2209751 Rabbit polyclonal anti-DsRed Clontech 632496; RRID: AB_10013483 Rabbit polyclonal anti-PKCγ Santa cruz sc-211; RRID: AB_632234 Rat monoclonal anti-Ctip2 Abcam ab18465; RRID: AB_10015215 Mouse monoclonal anti-NeuN Millipore MAB377; RRID: AB_2298772 Guinea pig polyclonal anti-Vglut1 Millipore AB5905; RRID: AB_2301751 Bacterial and Virus Strains PRV152 L. Enquist (Princeton Univ) N/A PRV614 L. Enquist (Princeton Univ) N/A AAV1-Syn-EGFP-Cre Penn vector core AV-1-PV1848 AAV1-CAG-tdTomato Penn vector core AV-1-PV3365 EnvA-SADΔG-mcherry E. Callaway and Gene Transfer, Targeting and Therapeutics Core (Salk Inst; Osakada et al., 2011 ) Addgene #32636 AAV1-Syn-FLEX-TVA-2A-EGFP-2A-RVG UNC vector core Addgene #52473 AAV1-CAG-ChR2(H134R)-mCherry Penn vector core AV-1–20938M AAV8-hSyn-DIO-hM4D(Gi)-mCherry UNC vector core Addgene #44362 Chemicals, Peptides, and Recombinant Proteins Green Retrobeads IX Lumafluor N/A Biotinylated dextran amine (10,000MW) Invitrogen D-1956 Fluorogold Fluorochrome N/A Clozapine-N-oxide Sigma-Aldrich C0832–5MG Experimental Models: Organisms/Strains Mouse: Rbp4-Cre GENSAT, MMRRC MMRRC:031125-UCD; MGI:4367067 Mouse: En1-Cre A. Joyner (Memorial Sloan Kettering Cancer Center; Kimmel et al., 2000 ) MGI:2446434 Mouse: Chx10-Cre Azim et al., 2014 N/A Mouse: Chat-Cre The Jackson Laboratory JAX# 006410; MGI: 5475195 Mouse: Ptf1a-Cre C. Wright(Vanderbilt Univ.

Techniques: Plasmid Preparation, Recombinant, Software

a , Module crosscorrelograms for sessions in different rooms (A1×B1) at incremental degrees of rotation in rat #26718 (select number of rotations shown here for visualization purposes; see for additional rotations) (as in , right). Asterisks denote overall maximum for each module. b , Maximum correlation between 3D stacks of rate maps of sessions A1 and B1 (with cell identity along the z-axis) as a function of rotation for each module in rat #26718. Maximum correlation refers to the highest value within the crosscorrelation matrix at each rotation. Note that the rotation was nearly identical across modules. c , Rotation of each simultaneously recorded module between sessions (38 session pairs in 10 rats, as in ). Lines connect data from a single recording. A fourth module was recorded in 4 session pairs. Its rotation did not differ from the other simultaneously recorded modules . d , Difference in rotation between simultaneously recorded module pairs (n = 104 module pairs, each pair indicated by a circle). Red crosses indicate medians. Dashed gray line at 90° represents chance. e , Gray lines show coherent rotation between sessions for grid, head direction (HD), and border cells (n = 22 experiments). Lines connect data from a single recording.

Journal: bioRxiv

Article Title: Functional independence of entorhinal grid cell modules enables remapping in hippocampal place cells

doi: 10.1101/2025.09.24.677985

Figure Lengend Snippet: a , Module crosscorrelograms for sessions in different rooms (A1×B1) at incremental degrees of rotation in rat #26718 (select number of rotations shown here for visualization purposes; see for additional rotations) (as in , right). Asterisks denote overall maximum for each module. b , Maximum correlation between 3D stacks of rate maps of sessions A1 and B1 (with cell identity along the z-axis) as a function of rotation for each module in rat #26718. Maximum correlation refers to the highest value within the crosscorrelation matrix at each rotation. Note that the rotation was nearly identical across modules. c , Rotation of each simultaneously recorded module between sessions (38 session pairs in 10 rats, as in ). Lines connect data from a single recording. A fourth module was recorded in 4 session pairs. Its rotation did not differ from the other simultaneously recorded modules . d , Difference in rotation between simultaneously recorded module pairs (n = 104 module pairs, each pair indicated by a circle). Red crosses indicate medians. Dashed gray line at 90° represents chance. e , Gray lines show coherent rotation between sessions for grid, head direction (HD), and border cells (n = 22 experiments). Lines connect data from a single recording.

Article Snippet: These markers were tracked at 120 Hz with a 3D motion capture system (six overhead OptiTrack Flex 13 cameras and Motive recording software).

Techniques: